The Rate of Hydrolysis of Sucrose Essay -- Biology Science Experiments

The Rate of Hydrolysis of SucroseHypothesis - The digestion of sucrose into glucose and fructose iscarried out by the presence of the enzyme Sucrase. The exercise of theenzyme allow for be greatest at its optimum temperature since at thistemperature the enzymes tertiary structure will be correct to altogetherowthe formation of the substratum-enzyme complex right away and efficiently.This should be at 37C (body temperature), which is the temperature ofthe ileum where Sucrase is secreted and naturally functions.The first thing to say about enzymes is that they are proteins andthey are found in all types of organisms from humans to viruses. Theyfunction in the body as catalysts. In other excogitates they speed up therate of chemical replys in the body. Enzymes actually speed upchemical reactions by a factor of about 1 million. Enzymes are organiccatalysts. Without enzymes the metabolism of an organism would be tooslow for the organism to survive. The word enzyme actually mean val ues inyeast as they were first discovered in these micro-organisms. Enzymesare found inside cells (intracellular) and outside cells (extracellular). Intracellular enzymes are for example, those that disciplinemetabolism while extra cellular enzymes are for example, gut digestivejuices and the enzymes secreted by bacteria, which digest their foodoutside the body then reabsorb the products. Enzymes hunt down onparticular organic chemicals, these are called substrates. So ineffect food is the substrate of digestive enzymes. Each enzyme isspecific to one or a group of particular substrates. The majority ofthe reactions that occur in living organisms are enzyme-controlled.Without enzymes toxins would soon build up and the supply ofrespiratory substrate would decrease. Enzymes are proteins and therefromhave a specific shape. They are therefore specific in the reactionsthat they catalyse - one enzyme will react with molecules of onesubstrate. The site of the reaction occurs in an area o n the surfaceof the protein called the active site. Since the active site for allmolecules of one enzyme will be made up of the same arrangement of amino acids, it has a highly specific shape. Generally, there is onlyone active site on each enzyme molecule and only one type of substratemolecule will fit into it. Lock and key hypothesis description of thespecificity of t... ... well as this the final colour was defined.Results my results from then on were much much reliable and theaverage was easier to plot on a graph drawing my expected curve. Thefirst colour change was a loss of the base pick apart colour and the finalcolour change is a measurable blue.Throughout all these preliminary experiments and the final one alllaboratory safety precautions will be observed. To check thereliability of my results I will take a mean average of all 3experiments I finally did. Using the mean of all the experiments Ithen will work out the standard deviation of the primary colour changeand the final colour change.On the graphs the X axis is numbered 1-6, this corresponds to theSucrase Concentration 0.0%-2.5%.Below is the preliminary experiment graph for primary colour change at0-5% solution.The line slopes down as the reaction time decreases in relation to theconcentration. This was the graph of the preliminary experiment aftermaking the changes due to evidence from preliminary experiment 1 and2.Prediction I predict that the higher the concentration the faster thereaction due to induced fit and lock and key hypothesis.